Serotyping of Ureaplasma urealyticum by immunoperoxidase assay
نویسندگان
چکیده
منابع مشابه
Development of an enzyme-linked immunosorbent assay for serotyping ureaplasma urealyticum strains using monoclonal antibodies.
Ureaplasma urealyticum comprises 14 serotypes. The existing serotyping methods all use polyclonal antibodies. These methods are time-consuming and labor-intensive, and they cannot always be performed on primary isolates; in addition, the results are difficult to interpret. We developed a new enzyme-linked immunosorbent assay (ELISA) method using serotype-specific monoclonal antibodies (MAbs) to...
متن کاملExpanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans.
For the last decade the serological heterogeneity of strains of Ureaplasma urealyticum isolated from humans has been represented by eight serotypes. We have identified two additional serotypes, strains Vancouver and Western. Using a modified metabolic inhibition test and a colony indirect epifluorescence method, we tested antisera to the above 10 types against the 11 members of the independent ...
متن کاملInfection of the epididymis by Ureaplasma urealyticum.
Ureaplasma urealyticum organisms (ureaplasmas) were isolated from the urethra and epididymal aspirate of a man aged 24 who had acute right sided epididymitis. No other microorganisms were detected, and he had no chlamydial antibody response. A fourfold antibody response to the epididymal ureaplasma isolate was detected by two methods, however, and the patient responded clinically to doxycycline...
متن کاملModified metabolic inhibition test for serotyping strains of Ureaplasma urealyticum (T-strain Mycoplasma).
Antisera prepared against the eight recognized serotypes of Ureaplasma urealyticum were tested against the homologous and heterologous antigens by a modified metabolic inhibition test that used bromothymol blue broth in microtiter plates. The method gives clear endpoint determinations which are usually maintained on continued incubation. Antisera against serotype 8 showed cross-reactions with t...
متن کاملCharacteristics of Ureaplasma urealyticum urease.
Sonication of Ureaplasma urealyticum cells grown in a dialysate growth medium effectively separated the cytoplasmic fraction from the membrane fraction, with both fractions relatively free from exogenous contaminating proteins. The urease activity was associated with the cytoplasmic fraction, and the ureaplasmal urease exhibited a specific activity higher than that of crystalline jack bean urea...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1981
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.13.4.670-676.1981